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Vinylferrocene copolymers based biosensors for phenol derivatives
Author(s) -
Dursun Fuat,
Ozoner Seyda Korkut,
Demirci Ali,
Gorur Mesut,
Yilmaz Faruk,
Erhan Elif
Publication year - 2012
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.2688
Subject(s) - glycidyl methacrylate , polypyrrole , copolymer , amperometry , electrode , glutaraldehyde , covalent bond , methacrylate , biosensor , electrochemistry , pyrrole , materials science , polymer chemistry , chemistry , nuclear chemistry , polymer , organic chemistry , nanotechnology
BACKGROUND: Newly synthesized composite films of P(glycidyl methacrylate 85 ‐ co ‐vinylferrocene 15 )/Poly(glutaraldehyde)/ Polypyrrole [P(GMA 85 ‐ co ‐VFc 15 )/PGA/PPy] and Poly(3‐methylthienyl methacrylate 85 ‐ co ‐vinylferrocene 15 )/Polypyrrole [P (MTM 85 ‐ co ‐VFc 15 )/PPy] were used as matrices for tyrosinase based working electrodes. Direct covalent attachment of enzyme was carried out via the pendant epoxy groups of P(GMA 85 ‐ co ‐VFc 15 ) film, and the entrapment of enzyme was achieved for electrode containing P(MTM 85 ‐ co ‐VFc 15 ) film via electropolymerization of pyrrole in the presence of enzyme. The aim of the study is amperometric determination of various phenolics and investigation of the effect of interfacial interactions between enzyme and matrices on biosensor response. RESULTS: The lowest detection limit and the highest sensitivity for a P(GMA 85 ‐ co ‐VFc 15 ) based working electrode was found to be 0.113 µmol L −1 for 4‐methoxyphenol, 40 nA (µmol L −1 ) −1 for pyrocatechol, respectively. Results showed that sensitivities were at least 8500–55000 times higher than the results in previous P(GMA‐ co ‐VFc) related studies. CONCLUSION: Facilitated electron transfer was achieved by means of mediator incorporated in conductive composites of VFc based redox copolymers. The effect was greater when enzyme was covalently bonded via epoxy groups due to the proximity of enzyme, mediator and electrode surface. Results showed that a multifunctional surface was provided on electrodes since the suggested copolymers could mediate an electrochemical reaction, and the multifunctional surface was capable of coating with conductive PPy. Copyright © 2011 Society of Chemical Industry