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The quest for alternatives to microbial cellulase mix production: corn stover‐produced heterologous multi‐cellulases readily deconstruct lignocellulosic biomass into fermentable sugars
Author(s) -
Park SangHyuck,
Ransom Callista,
Mei Chuansheng,
Sabzikar Robab,
Qi Chunfang,
Chundawat Shishir,
Dale Bruce,
Sticklen Mariam
Publication year - 2011
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.2584
Subject(s) - cellulase , corn stover , cellulosic ethanol , cellulose , lignocellulosic biomass , trichoderma reesei , chemistry , biomass (ecology) , food science , biochemistry , fermentation , agronomy , biology
BACKGROUND: Production of cellulosic ethanol is still expensive compared with corn (maize) grain ethanol due to the high costs of bulk production of microbial cellulases. At least three cellulases including endo‐cellulase, exo‐cellulase and cellobiase are needed to convert cellulosic biomass into fermentable sugars. All these cellulases could be self‐produced within cells of transgenic bio‐energy crops. The production of heterologous Acidothermus cellulolyticus (E1) endo‐cellulase in endoplasmic reticulum and mitochondria of green tissues of transgenic corn plants was recently reported, and it was confirmed that the heterologous E1 converts cellulose into fermentable sugars. RESULTS: Biologically active A. cellulolyticus E1, Trichoderma reesei 1,4‐β‐cellobiohydrolases I (CBH I) exo‐cellulase and bovine rumen Butyrivibrio fibrisolvens cellobiase were expressed in corn plant endoplasmic reticulum (ER), apoplast (cell wall areas) and vacuole respectively. Results show that the ratio 1:4:1 (E1:CBH I:cellobiase) of crude heterologous cellulases is ideal for converting ammonia fiber explosion (AFEX) pretreated corn stover into fermentable sugars. CONCLUSIONS: Corn plants that express all three biologically active heterologous cellulases within their cellulosic biomass to facilitate conversion of pretreated corn stover into fermentable sugars is a step forward in the quest for alternatives to the present microbial cellulase mix production for cellulosic biofuels. Copyright © 2011 Society of Chemical Industry