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Engineering a mammalian super producer
Author(s) -
Dietmair Stefanie,
Nielsen Lars K.,
Timmins Nicholas E.
Publication year - 2011
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.2576
Subject(s) - context (archaeology) , productivity , synthetic biology , production (economics) , underpinning , metabolic engineering , biochemical engineering , product (mathematics) , yield (engineering) , microbiology and biotechnology , biology , engineering , computational biology , economics , biochemistry , paleontology , civil engineering , geometry , mathematics , materials science , metallurgy , macroeconomics , enzyme
Mammalian cells are the preferred host for the manufacture of a wide range of biopharmaceuticals, but production costs are high owing to low productivity. A range of rational engineering strategies have been pursued in order to increase volumetric product titres from mammalian cells, such as delaying apoptosis, manipulation of the cell cycle, and improving metabolism and protein processing. Unfortunately, outcomes from these strategies have been mixed, with few instances where significant improvements in product yield have been achieved. This article reviews and contrasts many of the engineering strategies attempted to date, highlighting the variability and context specificity in outcome. The paper argues that this is a reflection of the complexity of mammalian cells, and that a deeper understanding of the biology underpinning protein production for biotechnological purposes is required. Copyright © 2011 Society of Chemical Industry