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Esterification activities of non‐commercial lipases after pre‐treatment in pressurized propane
Author(s) -
Kuhn Graciele,
Marangoni Marluci,
Freire Denise M. G.,
Soares Valéria Ferreira,
de Godoy Mateus Gomes,
de Castro Aline Machado,
Luccio Marco Di,
Treichel Helen,
Mazutti Marcio A.,
Oliveira Débora,
Oliveira J. Vladimir
Publication year - 2010
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.2376
Subject(s) - chemistry , propane , lipase , refinery , cabin pressurization , chromatography , biotransformation , enzyme , enzyme assay , bar (unit) , organic chemistry , materials science , composite material , physics , meteorology
Abstract BACKGROUND: The objective of this work was to assess the influence of propane pre‐treatment on the esterification activities of two non‐commercial lipases from Penicillium simplicissimum and Aspergillus parasiticus in the lyophilized and immobilized forms, evaluating the effect of pressure, exposure time and depressurization rate on the lipase activity by a full 2 3 experimental design. RESULTS: For both lyophilized and immobilized lipases from all sources evaluated, enhancement in residual activities after incubation in pressurized propane was observed, under several experimental conditions. The highest increment (427%) occurred with the lyophilized enzyme from P. simplicissimum was maintained pressurized at 30 bar for 1 h and then depressurized at the fastest rate (20 bar min −1 ). CONCLUSION: The enzyme activity changes significantly depending on the enzyme source, its presentation form, and the experimental pre‐treatment conditions investigated, such as exposure time, depressurization rate and pressure. The results obtained here can contribute as a basis for the selection of appropriate operational conditions, so that these catalysts can be applied in biotransformation reactions. Copyright © 2010 Society of Chemical Industry