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Different combinations of salts affect the growth and bacteriocin production by Lactobacillus salivarius CRL 1328
Author(s) -
Juárez Tomás María Silvina,
Bru Elena,
NaderMacías María Elena
Publication year - 2010
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.2271
Subject(s) - bacteriocin , lactobacillus salivarius , chemistry , food science , sodium acetate , factorial experiment , probiotic , lactobacillus , bacteria , sodium , microbiology and biotechnology , chromatography , biology , fermentation , antimicrobial , organic chemistry , statistics , genetics , mathematics
BACKGROUND: The culture medium for optimal growth of vaginal Lactobacillus salivarius CRL 1328 is different from that for optimal bacteriocin production. To simultaneously obtain high amount of biomass and bacteriocin of this microorganism, the effects of different basal culture media and salts on both responses were evaluated. The study was performed by using a complete factorial experimental design 2 6 , with central points. Sixty‐four different growth media, which resulted from the combinations of two basal culture media and two concentrations of five salts (ammonium citrate, sodium acetate, MgSO 4 , MnSO 4 , and K 2 HPO 4 ) were assayed. RESULTS: Only the addition of MnSO 4 to each culture medium significantly stimulated the growth of L. salivarius . The presence of sodium acetate or MgSO 4 stimulated the bacteriocin production, while MnSO 4 and K 2 HPO 4 exerted an inhibitory effect. However, the simultaneous addition of MnSO 4 and sodium acetate to both basal culture media allowed high bacteriocin levels to be reached, attenuating the inhibitory effect of Mn 2+ . CONCLUSIONS: The application of a complete experimental design contributed to simultaneous optimization of the biomass and bacteriocin production of L. salivarius CRL 1328. The results obtained are potentially applicable to the technological production of probiotic bacteria and antagonistic substance to be included in a probiotic pharmaceutical product. Copyright © 2009 Society of Chemical Industry