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In vitro synthesis of polyhydroxyalkanoate catalyzed by class II and III PHA synthases: a useful technique for surface coatings of a hydrophobic support with PHA
Author(s) -
Sato Shun,
Minato Masayuki,
Kikkawa Yoshihiro,
Abe Hideki,
Tsuge Takeharu
Publication year - 2010
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.2261
Subject(s) - polyhydroxyalkanoates , highly oriented pyrolytic graphite , materials science , chemical engineering , coating , biopolymer , graphite , contact angle , in vitro , polymer chemistry , nanotechnology , chemistry , polymer , biochemistry , composite material , bacteria , genetics , engineering , biology
In vitro synthesis of polyhydroxyalkanoates (PHAs) on a hydrophobic support, i.e. highly oriented pyrolytic graphite (HOPG), was performed using class II PHA synthase (PhaC1 Pp ) from Pseudomonas putida and class III PHA synthase (PhaEC Av ) from Allochromatium vinosum . Using PhaC1 Pp and 3‐hydroxyoctanoyl‐CoA, a poly(3‐hydroxyoctanoate) [P(3HO)] film was formed on the hydrophobic support with a thickness of a few nanometers, as revealed by atomic force microscopy (AFM). A poly(3‐hydroxybutyrate) [P(3HB)] film was also formed using PhaEC Av and 3‐hydroxybutyryl‐CoA. AFM observations of the HOPG surface during P(3HB) film formation revealed that polymerized P(3HB) covered HOPG surface within the reaction time, resulting in the formation of a homogeneous ultra‐thin film. This method of ultra‐thin PHA film formation on a hydrophobic support may be applicable to the surface‐coating technique of materials with biodegradable, bioabsorbable, and biocompatible PHAs. Copyright © 2009 Society of Chemical Industry