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Kinetics of thermal inactivation of transglutaminase from a newly isolated Bacillus circulans BL32
Author(s) -
de Souza Claucia Fernanda Volken,
Faccin Débora Jung Luvizetto,
Mertins Omar,
Heck Júlio Xandro,
da Silveira Nádya Pesce,
Secchi Argimiro Resende,
Ayub Marco Antônio Záchia
Publication year - 2009
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.2201
Subject(s) - bacillus circulans , kinetics , tissue transglutaminase , chemistry , enthalpy , enzyme , enzyme kinetics , activation energy , biochemistry , stereochemistry , thermodynamics , active site , physics , quantum mechanics
BACKGROUND: This paper describes the modeling of the kinetics of thermal inactivation of transglutaminase (TGase) from a newly isolated Bacillus circulans BL32, isolated from the Amazon environment. The purified enzyme was incubated at temperatures ranging from 30 to 70 °C and values of the thermodynamic inactivation parameters, such as activation energy (Δ E ), activation enthalpy (Δ H ), activation entropy (Δ S ), and free energy (Δ G ) for thermal inactivation, were calculated. RESULTS: The kinetics of TGase thermo‐inactivation followed a Lumry–Eyring model. The enzyme was very stable up to 50 °C, with approximately 50% of activity remaining after heating for 12 h. It was completely inactivated by incubation at 70 °C for 2 min. Δ E for TGase was 350.5 kJ mol −1 . Δ H and Δ S for thermo‐inactivation of the TGase were 347.8 kJ mol −1 and 744 J mol −1 K −1 at 50 °C, respectively. Dynamic light scattering measurements suggest that the thermal inactivation of this microbial TGase can be partially attributed to the formation of aggregates. CONCLUSION: These results provide useful information about the thermal characteristics of the microbial TGase from B. circulans BL32 and indicate that this enzyme could be a good candidate for industrial applications. Copyright © 2009 Society of Chemical Industry