A simple method for biocatalyst immobilization using PVA‐based hydrogel particles | Zendy

Fernandes Pedro | Zendy; Marques Marco P.C. | Zendy; Carvalho Filipe | Zendy; Cabral Joaquim M.S. | Zendy

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A simple method for biocatalyst immobilization using PVA‐based hydrogel particles

Author(s) -

Fernandes Pedro,

Marques Marco P.C.,

Carvalho Filipe,

Cabral Joaquim M.S.

Publication year - 2009

Publication title -

journal of chemical technology and biotechnology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.64

H-Index - 117

eISSN - 1097-4660

pISSN - 0268-2575

DOI - 10.1002/jctb.2080

Subject(s) - inulinase , biocatalysis , chemistry , polyethylene glycol , yield (engineering) , extrusion , hydrolysis , peg ratio , michaelis–menten kinetics , immobilized enzyme , chromatography , invertase , biotransformation , chemical engineering , sucrose , materials science , enzyme assay , organic chemistry , enzyme , catalysis , composite material , ionic liquid , finance , economics , engineering

BACKGROUND: The aim of this study was to evaluate the feasibility of enzyme immobilization in PVA particles through extrusion of LentiKat ® Liquid in polyethylene glycol. Inulinase, with invertase activity for sucrose hydrolysis, was used as model system. RESULTS: Inulinase was effectively immobilized in PVA particles. The pH optimum of the enzyme activity was broadened for lower pH values. Mechanical instability of the PVA under prolonged incubation above 55 °C was observed. A 1.8‐fold increase in the apparent K M (Michaelis constant) suggests diffusion limitations as a result of immobilization. The immobilized biocatalyst exhibited considerable operational stability, since a decrease of roughly 10% in the product yield after 24 h biotransformation runs was observed in trials performed at 50 °C, following 20 repeated, consecutive batches. CONCLUSION: The results obtained highlight the potential of PVA‐based particles obtained through extrusion into PEG for the production of suitable biocatalysts for application in large‐scale processes. Copyright © 2008 Society of Chemical Industry

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