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Sonocatalytic damage of bovine serum albumin (BSA) under ultrasonic irradiation by mixed TiO 2 /SiO 2 powder
Author(s) -
Wang Jun,
Ding Na,
Zhang Zhaohong,
Wang Shixian,
Guo Ying,
Liu Bin,
Zhang Yuanyuan,
Zhang Xiangdong
Publication year - 2009
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.2076
Subject(s) - irradiation , catalysis , materials science , ultrasonic sensor , ionic strength , selectivity , bovine serum albumin , biomolecule , molecule , molar concentration , nuclear chemistry , chemical engineering , chemistry , aqueous solution , nanotechnology , chromatography , organic chemistry , nuclear physics , engineering , physics , acoustics
BACKGROUND: In order to effectively damage some biomolecules under ultrasonic irradiation, a mixed TiO 2 /SiO 2 powder with high catalytic activity and selectivity was used as a sonocatalyst. RESULTS: The mixed TiO 2 /SiO 2 powder heat treated at 450 °C for 30 min was adopted as a sonocatalyst and the damage to BSA molecules under ultrasonic irradiation was assessed. In addition, the effects of such variables as molar ratio of TiO 2 and SiO 2 , treatment temperature and time, ultrasonic irradiation time, catalyst amount, solution acidity, ionic nature and strength, ultrasonic irradiation power and D 2 O concentration on the damage to BSA molecules were studied by means of UV‐visible and fluorescence spectra. The results showed that the degree of damage was aggravated by an increase in ultrasonic irradiation time, catalyst amount, solution acidity, ultrasonic irradiation power and D 2 O concentration, but was reduced by an increase in ionic strength. CONCLUSION: The results indicated that the mixed TiO 2 /SiO 2 powder displayed higher activity and selectivity compared with nano‐sized TiO 2 and SiO 2 powders during the sonocatalytic damage of BSA. The extent of the damage decreased in the order TiO 2 /SiO 2 > nano‐sized TiO 2 > nano‐sized SiO 2 . These results are of great significance for applying sonocatalytic methods to treat tumours. Copyright © 2008 Society of Chemical Industry