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Development of a mathematical model for Bacillus circulans growth and alkaline protease production kinetics
Author(s) -
Subba Rao Chaganti,
Sathish Thadikamala,
Pendyala Brahamaiah,
Kumar Thella Prathap,
Prakasham Reddy Shetty
Publication year - 2009
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.2040
Subject(s) - bacillus circulans , fermentation , protease , chemistry , substrate (aquarium) , biomass (ecology) , bacterial growth , bioreactor , biochemistry , food science , mathematics , enzyme , bacteria , biology , organic chemistry , ecology , genetics
BACKGROUND: An unstructured mathematical model was developed to understand information on the relationship between Bacillus circulans growth and metabolism‐related protease production (using logistic and Luedeking–Piret equations respectively) in a batch reactor with respect to glucose consumption and fermentation time. The objective was to develop an indispensable tool for the optimisation, control, design and analysis of alkaline protease production. RESULTS: Biomass growth and enzyme production titres changed with a change in substrate concentration. Modelling analysis of biomass and enzyme production titres at different substrate concentrations revealed significant accuracy in terms of statistical consistency and robustness with respect to fermentation kinetic profiles. CONCLUSION: With the B. circulans strain used, an economic protease yield (2837 × 10 3 U g −1 ) with respect to biomass and glucose ratio was achieved at low substrate concentration (10 g L −1 ). The developed model could be effectively utilised for designing, controlling and up‐scaling the protease production process in high‐density fermentation in selected bioreactors with statistical consistency. Copyright © 2008 Society of Chemical Industry