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Biomonitoring of biosurfactant production by green fluorescent protein‐marked Bacillus subtilis W1012
Author(s) -
das Neves Luiz Carlos Martins,
Kobayashi Márcio Junji,
Rodrigues Thaís Miranda,
Converti Attilio,
Penna Thereza Christina Vessoni
Publication year - 2009
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.2014
Subject(s) - bacillus subtilis , green fluorescent protein , bioremediation , strain (injury) , food science , recombinant dna , bacillaceae , chemistry , fluorescence , biomass (ecology) , bacillales , biochemistry , biology , microbiology and biotechnology , bacteria , gene , genetics , physics , anatomy , quantum mechanics , agronomy
BACKGROUND: Biosurfactant production was investigated using two strains of Bacillus subtilis , one being a reference strain ( B. subtilis 1012) and the other a recombinant of this ( B. subtilis W1012) made able to produce the green fluorescent protein (GFP). RESULTS: Batch cultivations carried out at different initial levels of glucose ( G 0 ) in the presence of 10 g L −1 casein demonstrated that the reference strain was able to release higher levels of biosurfactants in the medium at 5.0≤ G 0 ≤10 g L −1 ( B max = 104–110 mg L −1 ). The recombinant strain exhibited slightly lower levels of biosurfactants ( B max = 90–104 mg L −1 ) but only at higher glucose concentrations ( G 0 ≥ 20 g L −1 ). Under these nutritional conditions, the fluorescence intensity linked to the production of GFP was shown to be associated with the cell concentration even after achievement of the stationary phase. CONCLUSION: The ability of the genetically‐modified strain to simultaneously overproduce biosurfactant and GFP even at low biomass concentration makes it an interesting candidate for use as a biological indicator to monitor indirectly the biosurfactant production in bioremediation treatments. Copyright © 2008 Society of Chemical Industry