Response surface methodology as a tool to study the lipase‐catalyzed synthesis of betulinic acid ester | Zendy

Yasin Yamin | Zendy; Basri Mahiran | Zendy; Ahmad Faujan | Zendy; Salleh Abu Bakar | Zendy

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Response surface methodology as a tool to study the lipase‐catalyzed synthesis of betulinic acid ester

Author(s) -

Yasin Yamin,

Basri Mahiran,

Ahmad Faujan,

Salleh Abu Bakar

Publication year - 2008

Publication title -

journal of chemical technology and biotechnology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.64

H-Index - 117

eISSN - 1097-4660

pISSN - 0268-2575

DOI - 10.1002/jctb.1858

Subject(s) - betulinic acid , response surface methodology , candida antarctica , lipase , yield (engineering) , chemistry , catalysis , central composite design , chromatography , desorption , organic chemistry , enzyme , adsorption , materials science , biology , genetics , metallurgy

BACKGROUND: The synthesis of betulinic acid ester using betulinic acid and oleyl alcohol catalyzed by Novozym 435 (immobilized Candida antarctica lipase) was carried out. Response surface methodology (RSM) based on a five‐level, three‐variable, central composite rotatable design (CCRD) was employed to evaluate the interactive effects of various parameters. The parameters were reaction time (8–16 h), temperature (20–60 °C) and enzyme amount (120–160 mg). RESULTS: Simultaneously increasing reaction time, temperature and amount of enzyme increased the yields of betulinic acid ester produced. CONCLUSION: The optimum conditions derived via RSM for the reaction were reaction time of 10.2 h, temperature of 53.1 °C and enzyme amount of 138 mg. The actual experimental yield was 48.5% under optimum conditions, which compared well with the maximum predicted value of 47.6%. Copyright © 2008 Society of Chemical Industry

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