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Colloid deposition experiments as a diagnostic tool for biomass attachment onto bioproduct adsorbent surfaces
Author(s) -
Tari Canan,
Vennapusa RamiReddy,
Cabrera Rosa B,
FernándezLahore Marcelo
Publication year - 2008
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.1852
Subject(s) - dlvo theory , deposition (geology) , biomass (ecology) , chemical engineering , adsorption , colloid , chemistry , nanotechnology , materials science , chromatography , organic chemistry , geology , paleontology , oceanography , sediment , engineering
BACKGROUND: Detrimental processing conditions can be expected in any downstream operation where direct contacting between a crude feedstock and a reactive solid phase is supposed to occur. In this paper we have investigated the factors influencing intact yeast cells deposition onto anion and cation exchangers currently utilized for expanded‐bed adsorption of biotechnological products. The aim of this study was twofold: (a) to confirm previous findings relating biomass deposition with surface energetics according to the extended Derjaguin, Landau, Verwey and Overbeek theory (XDLVO) theory; and (b) to provide a simple experimental tool to evaluate biomass deposition onto process surfaces. RESULTS: Biomass deposition experiments were performed on an automated workstation utilizing a packed‐bed format. Two commercial ion exchangers intended for the direct capture of bioproducts in the presence of suspended biological particles were employed. Intact yeast cells in the late exponential phase of growth were selected as model bio‐colloids. Cell deposition was systematically evaluated as a function of fluid‐phase conductivity and quantitatively expressed as a biomass deposition parameter (α). CONCLUSION: α ≤0.15 was established as a criterion to reflect negligible biomass adhesion to the process support(s). Biomass deposition experiments further confirmed predictions made on the basis of free interfacial energy calculations as per the extended DLVO approach. Copyright © 2008 Society of Chemical Industry