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Purification of α‐toxin from Clostridium perfringens type A in PEG‐phosphate aqueous two‐phase systems: a factorial study
Author(s) -
Cavalcanti Maria TH,
Porto Tatiana S,
Neto Benicio B,
LimaFilho José L,
Porto Ana L F,
Pessoa Adalberto
Publication year - 2008
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.1798
Subject(s) - peg ratio , phosphate , factorial experiment , aqueous solution , chemistry , chromatography , partition coefficient , clostridium perfringens , polyethylene glycol , potassium phosphate , ethylene glycol , biochemistry , organic chemistry , biology , mathematics , statistics , finance , bacteria , economics , genetics
BACKGROUND: Purification of α‐toxin produced by Clostridium perfringens type A in aqueous two‐phase systems (ATPS) was studied with a full two‐level factorial design on two factors (concentrations of 8000 g mol −1 PEG and phosphate salt at pH 8.0), to estimate the influence of these factors on the purification results. RESULTS: The partition coefficient ( K ), purification factor ( PF ) and activity yield ( Y ) were strongly influenced by the PEG and phosphate concentrations. Raising the levels of the two factors increased these responses. The highest purification factor (5.7) was obtained with PEG and phosphate concentrations of 17.5% and 15%, respectively. CONCLUSION: These results support the proposal that polymer excluded volume and hydrophobic interactions are the factors that drive the α‐toxin in PEG/phosphate aqueous two‐phase systems. Copyright © 2008 Society of Chemical Industry