Premium
Liquid–liquid extraction of biomolecules: an overview and update of the main techniques
Author(s) -
Mazzola Priscila G,
Lopes Andre M,
Hasmann Francislene A,
Jozala Angela F,
Penna Thereza CV,
Magalhaes Perola O,
RangelYagui Carlota O,
Pessoa Jr Adalberto
Publication year - 2008
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.1794
Subject(s) - biomolecule , extraction (chemistry) , aqueous solution , polymer , chemistry , chromatography , nanotechnology , liquid–liquid extraction , phase (matter) , materials science , computer science , organic chemistry
Two‐phase systems can be exploited in separation science for the extraction/purification of desired biomolecules. This article reviews recent experimental work on the use of aqueous two‐phase polymer systems, two‐phase aqueous micellar systems and reversed micellar systems for the extraction/purification of biomolecules. The experimental partitioning behavior of different biomolecules is reviewed and new results with nisin and lipopolysaccharides are presented. An extensive description of each system is presented, based on the biomolecules behavior and the tools employed to improve their extraction and purification. Copyright © 2007 Society of Chemical Industry