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Improvement of the catalytic performance of lignin peroxidase in reversed micelles
Author(s) -
Lan Jing,
Zhang Yun,
Huang Xirong,
Hu Ming,
Liu Weifeng,
Li Yuezhong,
Qu Yinbo,
Gao Peiji
Publication year - 2008
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.1783
Subject(s) - chemistry , catalysis , pulmonary surfactant , micelle , peroxidase , lignin , nuclear chemistry , ether , membrane , lignin peroxidase , guaiacol , chromatography , organic chemistry , enzyme , biochemistry , aqueous solution
BACKGROUND: Anionic surfactant sodium bis (2‐ethylhexyl) sulfosuccinate (AOT) had an inhibiting effect on lignin peroxidase (LiP). To improve the catalytic activity of LiP in an AOT reversed micelle in isooctane, nonionic surfactant polyoxyethylene lauryl ether (Brij30) was incorporated into the interfacial membrane. H 2 O 2 played dual roles in the LiP‐catalyzed oxidation of substrates. To obtain a sustainable high activity of LiP, a coupled enzymatic reaction, i.e. the glucose oxidase (GOD)‐catalyzed oxidation of glucose was used as an H 2 O 2 source. RESULTS: Owing to modification of the charge density of the interfacial membrane, the activity of LiP in an optimized AOT/Brij30 reversed micellar medium (χ B (the molar percentage of Brij30) = 0.53, ω 0 ([H 2 O]/([AOT] + [Brij30]) = 23, pH = 4.8) was 40 times that in a single AOT reversed micelle. Due to the controlled release of H 2 O 2 , the concentration of H 2 O 2 in the mixed reversed micellar medium was maintained at a moderately high level throughout, which made the LiP‐catalyzed oxidation of substrates proceed at a higher conversion rate than counterparts in which H 2 O 2 was supplied externally in one batch at the beginning of the reaction. Decolourization of two waterless‐soluble aromatic dyes (pyrogallol red and bromopyrogallol red) using LiP coupled with GOD in the medium also demonstrated that a higher decolourization percentage was obtained if H 2 O 2 was supplied enzymatically. CONCLUSION: The proposed measures (both physicochemical and biochemical) were very effective, giving significant improvement in the catalytic performance of LiP in a single AOT reversed micelle in isooctane, which helped to degrade or transform hydrophobic aromatic compounds with LiP in reversed micelles more efficiently. Copyright © 2007 Society of Chemical Industry