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Optimization of lipase production by Penicillium simplicissimum in soybean meal
Author(s) -
Vargas Gean D. L. P.,
Treichel Helen,
de Oliveira Debora,
Beneti Stéphani C.,
Freire Denise M. G.,
Di Luccio Marco
Publication year - 2008
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.1776
Subject(s) - lipase , food science , substrate (aquarium) , chemistry , soybean meal , soybean oil , moisture , biochemistry , enzyme , biology , organic chemistry , raw material , ecology
BACKGROUND: Lipase production by Penicillium simplicissimum using soybean meal as substrate has been investigated. A factorial design technique was used to evaluate the effects of incubation temperature, initial moisture of the meal and substrate supplementation with low cost supplements, on lipase production. Soybean oil and wastewater from a slaughterhouse, which is rich on oil and fat, corn steep liquor and yeast hydrolysate, were tested as supplementary carbon and nitrogen sources. RESULTS Cultivation conditions were optimized for the production of lipase by factorial design and response surface methodology. Results show that the microorganism produces very low protease activity (0.21 U gds −1 dry substrate), which helps to maximize lipase production. Soybean meal without supplements appears to be the best medium of those tested for lipase production by P. simplicissimum. CONCLUSION: This work showed that temperature and moisture are the factors that most strongly influence lipase production by P. simplicissimum using soybean meal as substrate. The growth conditions that optimize lipase production are 27.5 °C using substrate with 550 g kg −1 of initial moisture. In optimum conditions lipase activity of 30 U gds −1 dry substrate was obtained. Copyright © 2007 Society of Chemical Industry