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Comparative study of poly(vinyl alcohol)‐based support materials for the immobilization of glucose oxidase
Author(s) -
Wong FuiLing,
AbdulAziz Azila
Publication year - 2008
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.1774
Subject(s) - vinyl alcohol , glucose oxidase , membrane , alcohol , materials science , sol gel , alcohol oxidase , polymer chemistry , hybrid material , chemical engineering , chemistry , nuclear chemistry , enzyme , organic chemistry , composite material , nanotechnology , polymer , biochemistry , pichia pastoris , recombinant dna , engineering , gene
BACKGROUND: The performances of four types of glucose oxidase (GOD) immobilization materials based on poly(vinyl alcohol) (PVA) were compared. The matrices of interest were chemically‐linked PVA, freeze‐thawed PVA cryogel, tetramethoxysilane (TMOS) sol‐gel‐PVA hybrid material, and alumina sol‐gel‐PVA hybrid material. RESULTS: Overall, the membranes showed good sensitivity except for the chemically cross‐linked PVA. However, the main differences with the enzyme immobilization methods were enzyme leakage and values of K m app . CONCLUSION: Freeze‐thawed PVA‐GOD membranes and TMOS‐PVA, which showed satisfactory sensitivity and adequate value of K m app , were quite promising as support materials for immobilizing GOD. Copyright © 2007 Society of Chemical Industry