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Optimization of nitrilase production from a new thermophilic isolate
Author(s) -
Khandelwal Amit Kumar,
Nigam Vinod Kumar,
Choudhury Bijan,
Mohan Medicherla Krishna,
Ghosh Purnendu
Publication year - 2007
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.1721
Subject(s) - nitrilase , chemistry , thermophile , benzonitrile , hydrolysis , inducer , enzyme , strain (injury) , bioreactor , organic chemistry , biochemistry , biology , gene , anatomy
A new thermostable nitrilase‐producing isolate identified as Streptomyces sp. MTCC 7546 has been studied extensively for the optimization of enzyme production operating in batch mode. The benzonitrile was observed as inducer of nitrilase production. The isolate showed maximum nitrilase production after 24 h of incubation at optimal conditions. The strain grows well on a variety of carbon sources and produces the nitrilase that catalyses the hydrolysis of nitriles to acids without formation of amides. The enzyme is mostly active against mono‐ and di‐aliphatic nitriles (10 mmol L −1 ) at pH of 7.4 and at a temperature of 50 °C. Copyright © 2007 Society of Chemical Industry