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Biodegradation of 2‐fluorobenzoate in upflow fixed bed bioreactors operated with different growth support materials
Author(s) -
Emanuelsson Maria AE,
Henriques Isabel S,
Ferreira Jorge Ruben M,
Castro Paula ML
Publication year - 2006
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.1565
Subject(s) - biodegradation , bioreactor , temperature gradient gel electrophoresis , chemistry , population , microbial population biology , adsorption , biofilm , chromatography , environmental chemistry , pulp and paper industry , bacteria , biology , organic chemistry , biochemistry , genetics , demography , 16s ribosomal rna , sociology , engineering , gene
Three upflow fixed bed bioreactors treating an aqueous stream containing 2‐fluorobenzoate were operated for a period of 7 months, during which they were exposed to high organic loading rates and starvation. The reactors contained granular activated carbon (GAC), polyethylene (PE) particles and expanded clay (EC) respectively as growth support for microbial biofilms. The performance of the reactors was compared and the biofilm microbial population was followed by cell counting and denaturing gradient gel electrophoresis (DGGE). The reactor containing GAC always had 100% removal efficiency owing to the adsorption properties of the material combined with biodegradation. The GAC reactor also recovered better after starvation periods in the sense that it showed more stable behaviour than the reactors containing EC and PE. The highest biological elimination capacity was observed for the reactor containing EC, which reached 200 mg day −1 L −1 during reactor start‐up, but during long‐term operation the reactor containing GAC showed the highest biological elimination capacity, 140 mg day −1 L −1 . DGGE analysis indicated that starvation periods seemed to be responsible for shifts in the microbial population. Copyright © 2006 Society of Chemical Industry