Premium
Optimization of 1,3‐propanediol production by novel recombinant Escherichia coli using response surface methodology
Author(s) -
Zhang Xiaomei,
Li Yin,
Zhuge Bin,
Tang Xueming,
Shen Wei,
Rao Zhiming,
Fang Huiying,
Zhuge Jian
Publication year - 2006
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.1538
Subject(s) - response surface methodology , fermentation , escherichia coli , 1,3 propanediol , industrial fermentation , glycerol , propanediol , recombinant dna , yield (engineering) , yeast extract , chemistry , food science , biochemistry , chromatography , materials science , organic chemistry , metallurgy , gene
Response surface methodology was used to optimize 1,3‐propanediol production by a novel recombinant Escherichia coli JM109 (pHsh‐ dha B‐ yqh D). The optimal fermentation parameters for enhanced 1,3‐propanediol yield were found to be: glycerol 61.8 g L −1 , yeast extract 6.2 g L −1 , Vitamin B 12 0.049 g L −1 and fermentation time 30 h. Subsequent experimental trials confirmed the validity of the model. These optimal fermentation conditions in the cultivation flask culture led to a 1,3‐propanediol concentration of 43.1 g L −1 and a conversion rate of 69.7% (g g −1 ). A maximum 1,3‐propanediol concentration of 41.1 g L −1 was achieved in a 5 L fermenter using the optimized parameters. Copyright © 2006 Society of Chemical Industry