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Optimisation study of large‐scale enzymatic synthesis of oleyl oleate, a liquid wax ester, by response surface methodology
Author(s) -
Mat Radzi Salina,
Basri Mahiran,
Salleh Abu Bakar,
Ariff Arbakariya,
Mohammad Rosfarizan,
Abdul Rahman Mohammad Basyaruddin,
Raja Abdul Rahman Raja Noor Zaliha
Publication year - 2006
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.1404
Subject(s) - response surface methodology , oleyl alcohol , impeller , wax , central composite design , yield (engineering) , wax ester , chromatography , oleic acid , materials science , scale up , composite number , chemistry , chemical engineering , composite material , mechanical engineering , engineering , biochemistry , physics , classical mechanics
An optimisation study of the large‐scale enzymatic synthesis of a liquid wax ester from oleic acid and oleyl alcohol using Novozym 435 was carried out. Investigations were performed in batch mode with a stirred tank reactor (STR) with one multi‐bladed impeller. Response surface methodology (RSM) based on a five‐level, three‐variable central composite rotatable design (CCRD) was used to evaluate the interactive effects of various parameters. The parameters are amount of enzyme ( A ) (90–120 g), impeller speed ( B ) (100–400 rpm) and temperature ( C ) (40–60 °C). The optimum conditions derived via RSM at a fixed reaction time of 1 h were successfully optimised as A = 104 g, B = 388.0 rpm and C = 49.7 °C. The actual experimental yield was 96.7% under the optimum conditions, which compared well with the maximum predicted value of 97.6%. Copyright © 2005 Society of Chemical Industry
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