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Assessment of yeast viability under hyperbaric conditions through a modeling approach
Author(s) -
Coutinho João AP,
Belo Isabel,
Ferreira Eugénio C,
Coelho M Alice Z
Publication year - 2005
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.1248
Subject(s) - viability assay , methylene blue , saccharomyces cerevisiae , yeast , substrate (aquarium) , oxygen , chemistry , cell , food science , biology , biochemistry , organic chemistry , ecology , catalysis , photocatalysis
The effect of pressure (0.1–1.5 MPa) and oxygen concentration on the growth and viability of Saccharomyces cerevisiae was studied. Cell viability was assessed through the Methylene Blue staining method and the percentages of viable and non‐viable cells were estimated using digital image processing. A model taking into account cell viability was developed and used to describe the measured data. The model reveals the opposing effects between oxygen availability and the baric and oxidative stresses present on the system and can successfully describe not only the traditional biomass–product–substrate (X–P–S) evolution but also the ratio of viable cells with time. It is shown that cell viability in general is not constant during the experiments but strongly depends on the environment. Copyright © 2005 Society of Chemical Industry