Premium
Laccase improvement in submerged cultivation: induced production and kinetic modelling
Author(s) -
Tavares APM,
Coelho MAZ,
Coutinho JAP,
Xavier AMRB
Publication year - 2005
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.1246
Subject(s) - laccase , trametes versicolor , inducer , chemistry , biomass (ecology) , substrate (aquarium) , homogeneous , food science , pulp and paper industry , carbon source , metabolism , biochemistry , enzyme , biology , ecology , mathematics , combinatorics , engineering , gene
Improvement of laccase production by Trametes versicolor was made by employing different operational strategies. In the cell growth medium, various glucose concentrations were compared for improving laccase production. A clear and significant stimulation of enzyme production under carbon limitation was obtained. Copper, 2,5‐xylidine, and a phenolic mixture were also used as laccase inducers. A cooperative effect between the inducers on laccase production was identified. Mixtures of inducers produced higher laccase activities, reaching values of 5500 U dm −3 . Further productivity enhancement can be obtained using the inducers along with the carbon limitation strategy. It is shown that low laccase concentrations are obtained by a primary metabolism of T versicolor , and that phenolic compounds and carbon limitation induce a secondary metabolism, providing higher laccase concentrations. A mathematical model for laccase production based on a direct experimental measure of biomass, along with substrate consumption and enzymatic activity over time is proposed for non‐homogeneous fermentations of T versicolor . Copyright © 2005 Society of Chemical Industry