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Supercritical CO 2 extraction of accumulated capsidiol from biotic elicitor‐activated Capsicum annuum L fruit tissues
Author(s) -
Salgın Uǧur,
Üstün A Sülün,
Mehmetoǧlu Ülkü,
Çalımlı Ayla
Publication year - 2005
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.1114
Subject(s) - extraction (chemistry) , supercritical fluid , elicitor , yield (engineering) , supercritical fluid extraction , chemistry , pepper , solubility , supercritical carbon dioxide , chromatography , solvent , botany , horticulture , materials science , food science , biochemistry , biology , organic chemistry , metallurgy , gene
Abstract This work investigates the supercritical CO 2 extraction of capsidiol from pepper fruit tissues activated with Alternaria alternate (Fr) Keissler suspension culture as a biotic elicitor. Capsidiol production in the fruit tissue was markedly increased by the treatment with a biotic elicitor and reached its maximum level after 4 days of elicitation. The effects of separation parameters such as temperature, pressure, supercritical solvent flow rate, particle diameter and also initial capsidiol concentration were investigated on solubility, initial extraction rate and extraction yield. The optimal extraction conditions were obtained at the temperature of 40 °C, the pressure of 400 bar, the supercritical CO 2 flow rate of 2 cm 3 min −1 , and the average particle diameter of 116 µm. The results showed that the ratio of the supercritical CO 2 extraction yield to the organic solvent extraction yield was changed from 84 to 97 wt‐% depending on the initial capsidiol concentration. Copyright © 2004 Society of Chemical Industry