Premium
Optimization of polymerase chain reaction‐amplified conditions using the uniform design method
Author(s) -
Cao Yang,
Zheng Yuanyuan,
Fang Baishan
Publication year - 2004
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.1078
Subject(s) - polymerase chain reaction , simulated annealing , annealing (glass) , klebsiella pneumoniae , reaction conditions , polymerase chain reaction optimization , materials science , biological system , chemistry , computational biology , gene , biology , mathematics , mathematical optimization , escherichia coli , biochemistry , nested polymerase chain reaction , composite material , catalysis
Polymerase chain reaction (PCR) is a key procedure in modern molecular biology but may be adversely affected by many factors and it is difficult to optimize reaction conditions; a technically simple and effective method to set up an optimal PCR‐amplified system is required. Uniform design (UD) is a method that enables many factors to be investigated. For example, the dhaT gene from Klebsiella pneumoniae was amplified and the optimization of the amplified parameters including concentration of Mg 2+ , annealing temperature, annealing time and number of cycles based on UD was reported. After only 12 experimental trials, an optimal PCR‐amplified result was obtained, demonstrating the value of UD for optimization of PCR. Copyright © 2004 Society of Chemical Industry