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Wet oxidation pre‐treatment of woody yard waste: parameter optimization and enzymatic digestibility for ethanol production
Author(s) -
Lissens Geert,
Klinke Helene,
Verstraete Willy,
Ahring Birgitte,
Thomsen Anne Belinda
Publication year - 2004
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.1068
Subject(s) - hemicellulose , cellulose , chemistry , lignin , cellulase , fermentation , enzymatic hydrolysis , hydrolysis , wet oxidation , food science , nuclear chemistry , organic chemistry , catalysis
Woody yard waste with high lignin content (22% of dry matter (DM)) was subjected to wet oxidation pre‐treatment for subsequent enzymatic conversion and fermentation. The effects of temperature (185–200 °C), oxygen pressure (3–12 bar) and addition of sodium carbonate (0–3.3 g per 100 g DM biomass) on enzymatic cellulose and hemicellulose (xylan) convertibility were studied. The enzymatic cellulose conversion was highest after wet oxidation for 15 min at 185 °C with addition of 12 bars of oxygen and 3.3 g Na 2 CO 3 per 100 g waste. At 25 FPU (filter paper unit) cellulase g −1 DM added, 58–67% and 80–83% of the cellulose and hemicellulose contained in the waste were converted into monomeric sugars. The cellulose conversion efficiency during a simultaneous saccharification and fermentation (SSF) assay at 10% DM was 79% for the highest enzyme loading (25 FPU g −1 DM) while 69% conversion efficiency was still reached at 15 FPU g −1 DM. Total carbohydrate recoveries were high (91–100% for cellulose and 72–100% for hemicellulose) and up to 49% of the original lignin and 79% of the hemicellulose could be solubilized during wet oxidation treatment and converted into carboxylic acids mainly (total carboxylic acids = 3.1–7.4% on DM basis). Copyright © 2004 Society of Chemical Industry