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Effect of adsorption characteristics of a modified cellulase on indigo backstaining
Author(s) -
Mamma Diomi,
Kalantzi Styliani A,
Christakopoulos Paul
Publication year - 2004
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/jctb.1033
Subject(s) - cellulase , papain , chemistry , cellulose , adsorption , chromatography , protease , digestion (alchemy) , biochemistry , enzyme , organic chemistry
The effect of limited proteolysis (digestion) of a commercial cellulase preparation (Ecostone ® L350) on backstaining with indigo was investigated. The influence of protease (papain) concentration on limited proteolysis of cellulase preparation was studied, applying different ratios of papain/cellulase (w/w). Changes in adsorption on Avicel cellulose of the non‐digested compared with the papain‐digested Ecostone ® L350 were examined using the Langmuir adsorption isotherm. The non‐digested Ecostone ® L350 exhibited stronger interaction to Avicel cellulose compared with the digested form, while the maximum efficiency of cellulase adsorption to Avicel cellulose decreased after digestion. When papain‐digested Ecostone ® L350 was applied on cotton fabrics during the dyeing procedure with indigo, a reduction of indigo backstaining was obtained compared with the non‐digested Ecostone ® L350. Copyright © 2004 Society of Chemical Industry