Premium
Generation of biohybrid implants using a multipotent human periodontal ligament cell line and bioactive core materials
Author(s) -
Ono Taiga,
Tomokiyo Atsushi,
Ipposhi Keita,
Yamashita Kozue,
Alhasan M Anas,
Miyazaki Yudai,
Kunitomi Yoshihiro,
Tsuchiya Akira,
Ishikawa Kunio,
Maeda Hidefumi
Publication year - 2021
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.30336
Subject(s) - periodontal fiber , cementum , core (optical fiber) , 3d bioprinting , materials science , multipotent stem cell , cell culture , biomedical engineering , implant , chemistry , tissue engineering , dentistry , stem cell , microbiology and biotechnology , biology , progenitor cell , medicine , surgery , composite material , genetics , dentin
We aimed to generate periodontal ligament (PDL) tissue‐like structures from a multipotent human PDL cell line using three‐dimensional (3D) bioprinting technology and to incorporate these structures with bioactive core materials to develop a new biohybrid implant system. After 3D bioprinting, single‐cell spheroids were able to form 3D tubular structures (3DTBs). We established three types of complexes using 3DTBs and different core materials: 3DTB‐titanium core (TIC), 3DTB‐hydroxyapatite core (HAC), and 3DTB without a core material (WOC). The expressions of PDL‐, angiogenesis‐, cementum‐, and bone‐related genes were significantly increased in the three complexes compared with monolayer‐cultured cells. Abundant collagen fibers and cells positive for the above markers were confirmed in the three complexes. However, more positive cells were detected in HAC than in WOC or TIC. The present results suggest that 3D‐bioprinted structures and hydroxyapatite core materials can function similarly to the PDL and may be useful for the development of a new biohybrid implant system.