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Nuclear and cytoplasmic quality of oocytes derived from serum‐free culture of secondary follicles in vitro
Author(s) -
Li Ang,
Wang HuaiXiu,
Wang Feng,
Fan LiHua,
Zhao ZhengHui,
Han Feng,
Li Jian,
Lei WenLong,
Zhou Qian,
Shi YaPing,
Song ChunYing,
Schatten Heide,
Sun QingYuan,
Guo XingPing
Publication year - 2021
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.30232
Subject(s) - follicular phase , antral follicle , andrology , oocyte , biology , follicular fluid , in vitro , meiosis , microbiology and biotechnology , parthenogenesis , in vitro fertilisation , folliculogenesis , in vitro maturation , embryogenesis , embryo , endocrinology , genetics , medicine , gene
In vitro culture of follicles is a promising technology to generate large quantities of mature oocytes and it could offer a novel option of assisted reproductive technologies. Here we described a 2‐dimensional follicular serum‐free culture system with 3‐dimensional effect that can make secondary follicles develop into antral follicles (78.52%), generating developmentally mature oocytes in vitro (66.45%). The oocytes in this serum‐free system completed the first meiosis; spindle assembly and chromosome congression in most oocytes matured from follicular culture were normal. However, these oocytes showed significantly lower activation and embryonic development rates, and their ability to produce Ca 2+ oscillations was also lower in response to parthenogenetic activation, after which a 2‐cell embryonic developmental block occurred. Oocytes matured from follicular culture displayed increased abnormal mitochondrial distribution and increased reactive oxygen species levels when compared to in vivo matured oocytes. These data are important for understanding the reasons for reduced developmental potential of oocytes matured from follicular culture, and for further improving the cultivation system.