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Melatonin alleviates LPS‐induced endoplasmic reticulum stress and inflammation in spermatogonial stem cells
Author(s) -
Yang Donghui,
Wei Yudong,
Lu Qizhong,
Qin Dezhe,
Zhang Mengfei,
Du Xiaomin,
Xu Wenjing,
Yu Xiuwei,
He Chen,
Li Na,
Peng Sha,
Li Guangpeng,
Hua Jinlian
Publication year - 2021
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.30088
Subject(s) - endoplasmic reticulum , inflammation , lipopolysaccharide , biology , apoptosis , unfolded protein response , microbiology and biotechnology , downregulation and upregulation , orchitis , immunology , male infertility , infertility , gene , medicine , genetics , pathology , pregnancy
Orchitis is one of the leading causes of male animal infertility and is associated with inflammatory reactions caused by the bacterium. It has been reported that there is a mutual coupling effect between endoplasmic reticulum stress (ERS) and inflammatory response. Our studies showed that lipopolysaccharide (LPS) could cause testicular damages, apoptosis, ERS, and inflammatory responses in spermatogonial stem cells (SSCs); ERS‐related apoptosis proteins were activated and the expression of ERS genes was significantly upregulated; meanwhile, the expression of Toll‐like receptor 4 and inflammation factors was apparently increased with LPS treatment. Moreover, melatonin (MEL) could rescue testicular damage, and significantly inhibited the expression of ERS‐related apoptosis genes, ERS markers, and inflammatory factors in SSCs and MEL played repairing and anti‐infection roles in LPS‐induced testicular damage. Therefore, MEL may be used as a drug to prevent and control bacterial infections in male reproductive systems. However, the specific molecular mechanism of MEL to resist ERS and inflammatory response remains to be further studied.