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PAX7 promotes CD49f‐positive dairy goat spermatogonial stem cells' self‐renewal
Author(s) -
Du Xiaomin,
Wu Siyu,
Wei Yudong,
Yu Xiuwei,
Ma Fanglin,
Zhai Yuanxin,
Yang Donghui,
Zhang Mengfei,
Liu Wenqing,
Zhu Haijing,
Wu Jiang,
Liao Mingzhi,
Li Na,
Bai Chunling,
Li Guangpeng,
Hua Jinlian
Publication year - 2021
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.29954
Subject(s) - biology , microbiology and biotechnology , stem cell , spermatogenesis , transcription factor , cell sorting , downregulation and upregulation , andrology , cell , gene , endocrinology , genetics , medicine
Spermatogenesis is a complex process that originates from and depends on the spermatogonial stem cells (SSCs). The number of SSCs is rare, which makes the separation and enrichment of SSCs difficult and inefficient. The transcription factor PAX7 maintains fertility in normal spermatogenesis in mice. However, for large animals, much less is known about the SSCs' self‐renewal regulation, especially in dairy goats. We isolated and enriched the CD49f‐positive and negative dairy goat testicular cells by magnetic‐activated cell sorting strategies. The RNA‐ sequencing and experimental data revealed that cells with a high CD49f and PAX7 expression are undifferentiated spermatogonia in goat testis. Our findings indicated that ZBTB16 (PLZF) , PAX7 , LIN28A , BMPR1B , FGFR1 , and FOXO1 were expressed higher in CD49f‐positive cells as compared to negative cells and goat fibroblasts cells. The expression and distribution of PAX7 in dairy goat also have been detected, which gradually decreased in testis tissue along with the increasing age. When the PAX7 gene was overexpressed in dairy goat immortal mGSCs‐I‐SB germ cell lines, the expression of PLZF, GFRα1, ID4, and OCT4 was upregulated. Together, our data demonstrated that there is a subset of spermatogonial stem cells with a high expression of PAX7 among the CD49f + spermatogonia, and PAX7 can maintain the self‐renewal of CD49f‐positive SSCs.