Premium
Vitamin C alleviates the senescence of periodontal ligament stem cells through inhibition of Notch3 during long‐term culture
Author(s) -
Yang Yan,
Wang Tao,
Zhang Sicheng,
Jia Sixun,
Chen Hong,
Duan Yufeng,
Wang Shikai,
Chen Guoqing,
Tian Weidong
Publication year - 2021
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.29930
Subject(s) - periodontal ligament stem cells , senescence , periodontal fiber , microbiology and biotechnology , regeneration (biology) , stem cell , cell culture , biology , chemistry , medicine , biochemistry , dentistry , alkaline phosphatase , enzyme , genetics
Periodontal ligament stem cells (PDLSCs), as potential “seed cells” for periodontal tissue repair and regeneration, require to be expanded in vitro for a large scale. Senescence of PDLSCs occurred during long‐term culture may compromise the therapeutic effects of PDLSCs. Medium supplements may be useful in antisenescence. However, the effects and mechanisms of vitamin C (Vc) treatment on PDLSCs during long‐term culture are still unclear. In this study, we identified that Vc‐treated PDLSCs cells maintained a slender morphology, higher growth rate and migration capacity, stemness, and osteogenic differentiation capability during a long‐term culture. Moreover, we also identified that Notch3 was significantly upregulated during the cell senescence, and Vc treatment alleviated the senescence of PDLSCs through inhibition of Notch3 during long‐term culture. In summary, Vc treatment suppressed PDLSCs senescence by reducing the expression of Notch3 and might be a simple and useful strategy to inhibit cellular senescence during the cell long‐term culture.