Premium
Enalapril and treadmill running reduce adiposity, but only the latter causes adipose tissue browning in mice
Author(s) -
Giori Isabele G.,
Magliano D'Angelo C.,
AlexandreSantos Beatriz,
Fernandes Tiago,
Oliveira Edilamar M.,
Vieira Carla P.,
ConteJunior Carlos A.,
Ceddia Rolando B.,
Nobrega Antonio C. L.,
Frantz Eliete D. C.
Publication year - 2021
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.29900
Subject(s) - endocrinology , medicine , enalapril , white adipose tissue , adipose tissue , browning , adipocyte , chemistry , muscle hypertrophy , thermogenin , thermogenesis , angiotensin converting enzyme , biochemistry , blood pressure
This study investigated whether regulation of the renin–angiotensin system (RAS) by enalapril and/or aerobic exercise training (AET) causes browning of the subcutaneous white adipose tissue (sWAT). C57BL/6 mice were fed either a standard chow or a high‐fat (HF) diet for 16 weeks. At Week 8, HF‐fed animals were divided into sedentary (HF), enalapril (HF‐E), AET (HF‐T), and enalapril plus AET (HF‐ET) groups. Subsequently, sWAT was extracted for morphometry, determination of RAS expression, and biomarkers of WAT browning. The HF group displayed adipocyte hypertrophy and induction of the classical RAS axis. Conversely, all interventions reduced adiposity and induced the counterregulatory RAS axis. However, only AET raised plasma irisin, increased peroxisome proliferator‐activated receptor‐γ coactivator‐1α, and uncoupling protein‐1 levels, and the expression of PR‐domain containing 16 in sWAT. Therefore, we concluded that AET‐induced sWAT browning was independent of the counterregulatory axis shifting of RAS in HF diet‐induced obesity.