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Comparative study between bone marrow mesenchymal stem cell and their conditioned medium in the treatment of rat model of Parkinsonism
Author(s) -
Abdelwahab Soha,
Elsebay Sara Abdel Gawad,
Fouli Gaber Manar,
AbdelHafez Sara Mohamed Naguib
Publication year - 2021
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.29872
Subject(s) - parkinsonism , pathology , substantia nigra , stem cell , mesenchymal stem cell , dopaminergic , bone marrow , neuropil , neural stem cell , biology , medicine , chemistry , dopamine , endocrinology , microbiology and biotechnology , central nervous system , disease
Parkinsonism is one of the most common aging neurodegenerative disorders. This study aims to compare the therapeutic effect of stem cell versus its conditioned medium in the Parkinsonism model. Parkinsonism was induced by daily subcutaneous injection of 0.5 mg/kg of rotenone dissolved in dimethyl sulfoxide for 28 days. Fifty rats were divided randomly into five groups: control, dimethyl sulfoxide, Parkinsonism, stem cell‐treated, and conditioned medium‐treated groups. Midbrain specimens were obtained for histological, immunohistochemical, and biochemical studies. Lewy bodies were observed in the Parkinsonism group in the dopaminergic neuron and neuropil as well. Almost all of the pathological changes were clearly ameliorated in both stem cell‐ and conditioned medium‐treated groups as confirmed by biochemical, histological, and immunohistochemical (anti‐nestin, anti‐glial fibrillary acidic protein, and anti‐α synuclein) studies. However, the conditioned medium showed more superior therapeutic effect establishing nearly the normal histological architecture of substantia nigra. These results may pave the future for using stem cell‐conditioned medium as a more convenient and effective adjuvant therapy in Parkinsonism and other neurodegenerative disorders.