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Steroid‐depleted endometriosis serum improves oocyte maturation in IVM systems
Author(s) -
Asghari Samira,
Nouri Mohammad,
Darabi Masoud,
Valizadeh Amir
Publication year - 2021
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.29834
Subject(s) - germinal vesicle , in vitro maturation , andrology , apoptosis , steroid , flow cytometry , oocyte , in vitro , endometriosis , chemistry , medicine , endocrinology , biology , hormone , immunology , embryo , biochemistry , microbiology and biotechnology
Abstract In vitro maturation (IVM) is a novel approach to overcome the adverse effects of human in vitro fertilization (IVF). The aim of the present study is to evaluate the effect of total and steroid‐depleted serum obtained from patients with endometriosis on IVM outcome as supplementation for this system. To this purpose, patients with endometriosis were selected according to in/excluding criteria. Germinal vesicles (GVs) and cumulus cells were treated with 10% of each serum. The expression levels of stearoyl CoA desaturase 1 (SCD 1) and cyclooxygenase‐2 (COX‐2) genes were evaluated by RT‐qPCR. Gas‐liquid chromatography and flow cytometry were performed to analyze fatty acids composition and apoptosis. The mRNA expression levels of SCD1 (2.47 fold) and COX‐2 (6.4 fold), and also the synthesis of oleate, linoleate, and arachidonate were increased (1.19, 1.06, and 2.37 folds, respectively) in cumulus cells treated with steroid‐depleted serum ( p < .05). The synthesis of palmitate, palmitoleate, and stearate (0.995, 0.67, and 0.7 folds, respectively) and also the rate of apoptosis were significantly decreased in these cells ( p < .05). Moreover, GVs cultured in steroid‐depleted group showed a significantly higher rate of maturation ( p < .001). Overall, our findings imply a new insight into the expansion of IVM system in oocytes development.