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Exosome‐mediated transfer of miR‐1260b promotes cell invasion through Wnt/β–catenin signaling pathway in lung adenocarcinoma
Author(s) -
Xia Yang,
Wei Ke,
Hu LiuQing,
Zhou CunRong,
Lu ZhiBin,
Zhan GenSheng,
Pan XiangLong,
Pan ChunFeng,
Wang Jun,
Wen Wei,
Xu Jing,
He ZhiCheng,
Huang ChenJun,
Chen Liang
Publication year - 2020
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.29578
Subject(s) - exosome , wnt signaling pathway , microvesicles , microrna , cell , biology , microbiology and biotechnology , cancer research , signal transduction , a549 cell , cell growth , catenin , gene , biochemistry
Increasing evidence confirms that exosome‐mediated transfer of microRNAs can influence cancer progression including tumor cell invasion, cell proliferation, and drug resistance via cell–cell communication. However, the potential role of exosomal‐miR‐1260b in lung adenocarcinoma (LAC) remains poorly understood. Thus, this study focused on investigating the function of exosomal‐miR‐1260b on cell invasion. Exosomal‐miR‐1260b was found to be higher in plasma of patients with LAC than that of healthy persons via quantitative real‐time polymerase chain reaction assay. The sensitivity and specificity of exosomal‐miR‐1260b (cutoff point: 2.027) were 72% and 86%, and area under the curve of 0.845 (95% CI = 0.772–0.922). Elevated expression of miR‐1260b in LAC tissues was positively correlated with exosomal‐miR‐1260b in plasma ( r = .642, p < .05). Furthermore, ceramide biosynthesis regulated exosomal‐miR‐1260b secretion. Exosome‐mediated transfer of miR‐1260b promoted A549 cell invasion and was still functional inside A549 cells. Moreover, exosomal‐miR‐1260b regulated Wnt/β–catenin signaling pathway by inhibiting sFRP1 and Smad4. This study identified a new regulation mechanism involving in cell invasion by exosome‐mediated tumor‐cell‐to‐tumor‐cell communication. Targeting exosome‐microRNAs may provide new insights into the diagnosis and treatment of LAC.