Premium
Muscle regeneration in adiponectin knockout mice showed early activation of anti‐inflammatory response with perturbations in myogenesis
Author(s) -
Mosele Francielle C.,
Bissi Ricci Rafael,
Abreu Phablo,
Rosa Neto José C.
Publication year - 2020
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.29547
Subject(s) - proinflammatory cytokine , myogenesis , adiponectin , adipokine , endocrinology , medicine , knockout mouse , microbiology and biotechnology , regeneration (biology) , biology , in vivo , chemistry , inflammation , skeletal muscle , leptin , receptor , obesity , insulin resistance , insulin
Activation, proliferation, and differentiation of satellite cells can be influenced by extracellular factors, such as adiponectin. This adipokine has been proposed as a regulator of in vitro myogenesis, but its action on in vivo regeneration is not still elucidated. We used C57BL/6 (wild‐type [WT]) and adiponectin knockout (AdKO) mice injured with barium chloride at periods of 3, 7, and 14 days after injury. The AdKO presented a higher number of centralized nuclei after 7 days, and a reduction in myogenic genes was observed after 3 days. Moreover, these mice presented an increase in anti‐inflammatory cytokines after 3 and 7 days, and an increase in the M2 gene marker and proinflammatory cytokines after 7 days. The WT demonstrated an increase in adiponectin messenger RNA after 7 days. These results demonstrate that adiponectin is important in tissue remodeling during regeneration and that its deficiency does not compromise the maturation of muscle fibers, due to an increase in anti‐inflammatory response; however, there is a possible impairment in proinflammatory response and an increase in centralized myonuclei.