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circRNA‐14723 promotes hepatocytes proliferation in rat liver regeneration by sponging rno‐miR‐16‐5p
Author(s) -
Guo Xueqiang,
Xi Lingling,
Li Lifei,
Guo Jianlin,
Jin Wei,
Chang Cuifang,
Zhang Jingbo,
Xu Cunshuan,
Chen Guangwen
Publication year - 2020
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.29473
Subject(s) - cell growth , microrna , flow cytometry , liver regeneration , cyclin d1 , cyclin e1 , biology , microbiology and biotechnology , long non coding rna , rna , cell cycle , cell , cancer research , gene , regeneration (biology) , biochemistry
Circular RNA (circRNA) is a subclass of noncoding RNA (ncRNA) detected within mammalian tissues and cells. However, its regulatory role during the proliferation phase of rat liver regeneration (LR) remains unreported. This study was designed to explore their regulatory mechanisms in cell proliferation of LR. The circRNA expression profile was detected by high‐throughput sequencing. It was indicated that 260 circRNAs were differentially expressed during the proliferation phase of rat LR. Among them, circ‐14723 displayed a significantly differential expression. We further explored its regulatory mechanism in rat hepatocytes (BRL‐3A cells). First, EdU, flow cytometry and western blot (WB) indicated that knocking down circ‐14723 inhibited BRL‐3A cells proliferation. Second, RNA‐Pulldown and dual‐luciferase report assay showed that circ‐14723 could sponge rno‐miR‐16‐5p. At last, WB showed that the reported target genes of rno‐miR‐16‐5p, CCND1, and CCNE1 were downregulated after knocking down circ‐14723. In conclusion, we found that circ‐14723 exerted a critical role in G1/S arrest to promote cell proliferation via rno‐miR‐16‐5p/CCND1 and CCNE1 axis in rat LR. This finding further revealed the regulatory mechanisms of circRNA on cell proliferation of LR, and might provide a potential target for clinical problems.