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TRPM2‐AS inhibits the growth, migration, and invasion of gliomas through JNK, c‐Jun, and RGS4
Author(s) -
Bao MeiHua,
Lv QiaoLi,
Szeto Vivian,
Wong Raymond,
Zhu SuZhen,
Zhang YingYing,
Feng ZhongPing,
Sun HongShuo
Publication year - 2020
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.29336
Subject(s) - downregulation and upregulation , western blot , trpm2 , glioma , cancer research , small interfering rna , c jun , gene expression , biology , cell growth , clone (java method) , messenger rna , microbiology and biotechnology , gene , rna , genetics , receptor , transcription factor , transient receptor potential channel
Gliomas are a group of brain cancers with high mortality and morbidity. Understanding the molecular mechanisms is important for the prevention or treatment of gliomas. The present study was to investigate the effects and mechanisms of long noncoding RNA TRPM2‐AS in gliomas proliferation, migration, and invasion. We first compared the levels of TRPM2‐AS in 111 patients with glioma to that of the normal control group by a quantitative polymerase chain reaction. The results indicated a significant increase of TRPM2‐AS in patients with glioma (2.43 folds of control, p = .0135). MTT methods, wound healing assays, transwell analysis, and clone formation analysis indicated the overexpression of TRPM2‐AS promoted the proliferation, migration, and invasion of U251 and U87 cells, while downregulation of TRPM2‐AS inhibited the cell proliferation, migration, and invasion significantly ( p < .05). To further uncover the mechanisms, bioinformatics analysis was conducted on the expression profiles, GSE40687 and GSE4290, from the Gene Expression Omnibus database. One hundred fifty‐six genes were differentially expressed in both datasets (FC > 2.0; p = .05). Among these differentially expressed genes, the level of RGS4 messenger RNA was drastically regulated by TRPM2‐AS. Further western‐blot analysis indicated the increase of RGS4 protein expression and decrease of p‐JNK/JNK and p‐c‐Jun/c‐Jun ratio after TRPM2‐AS overexpression. On the other hand, inhibition of TRPM2‐AS by small interfering RNA suppressed the expression of RGS4 and promoted the ratios of p‐JNK/JNK and p‐c‐Jun/c‐Jun. The present work indicated the mechanisms of the participation of TRPM2‐AS in the progression of gliomas might, at least partly, be related to JNK, c‐Jun, and RGS4. Our work provided new insights into the underlying mechanisms of glioma cellular functions.