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Retracted : Circular RNA circFNDC3B protects renal carcinoma by miR‐99a downregulation
Author(s) -
Chen Tao,
Yu Qinchao,
Shao Shixiu,
Guo Lei
Publication year - 2020
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.29316
Subject(s) - downregulation and upregulation , viability assay , mapk/erk pathway , kinase , janus kinase , western blot , stat protein , cell growth , stat3 , microbiology and biotechnology , signal transduction , biology , chemistry , cell , cancer research , biochemistry , gene
Abstract Various circular RNAs (circRNAs) have been reported to involve in carcinoma. This study explored the role and mechanism of circRNA circFNDC3B (circFNDC3B) in renal carcinoma (RC). The detection indicators in this paper were viability, colony, and migration, which respectively investigated by Cell Counting Kit‐8, colony formation, and migration assay. Reverse transcriptase quantitative polymerase chain reaction tested and cell transfection changed circFNDC3B and miR‐99a expression. Moreover, western blot tested relate‐proteins of proliferation, migration, and cell pathways were examined by western blot. circFNDC3B was upregulated at RC tissues. circFNDC3B enhanced cell viability, colony and migration, and miR‐99a mimic played reverse impacts. Furthermore, circFNDC3B negatively regulated miR‐99aand circFNDC3B restrained the janus kinase 1/signal transducer and activator of transcription 3 (JAK1/STAT3) and extracellular signal‐regulated kinase (ERK) kinase (MEK)/ERK pathways by miR‐99a downregulation. Overexpression of circFNDC3B enhanced cell viability, colony formation and migration by miR‐99a downregulation via JAK1/STAT3 and MEK/ERK pathways.