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Interplay between neutrophils and trophoblast cells conditions trophoblast function and triggers vascular transformation signals
Author(s) -
Calo Guillermina,
Sabbione Florencia,
Pascuali Natalia,
Keitelman Irene,
Vota Daiana,
Paparini Daniel,
Ramhorst Rosanna,
Parborell Fernanda,
Trevani Analía,
Leirós Claudia Pérez
Publication year - 2020
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.29247
Subject(s) - trophoblast , placentation , microbiology and biotechnology , biology , angiogenesis , placenta , immunology , fetus , cancer research , pregnancy , genetics
Normal placentation entails highly regulated interactions of maternal leukocytes with vascular and trophoblast cells to favor vascular transformation. Neutrophil activation and neutrophil extracellular trap (NET) formation associate with poor placentation and severe pregnancy complications. To deepen into the mechanisms of trophoblast–neutrophil interaction, we explored the effects of NETs on trophoblast cell function and, conversely, whether trophoblast cell‐derived factors condition neutrophils to favor angiogenesis and anti‐inflammatory signals required for fetal growth. NETs isolated from activated neutrophils hindered trophoblast cell migration. Trophoblast conditioned media prevented the effect as well as the vasoactive intestinal peptide (VIP) known to regulate trophoblast and neutrophil function. On the other hand, factors released by trophoblast cells and VIP shaped neutrophils to a proangiogenic profile with increased vascular endothelial growth factor synthesis and increased capacity to promote vascular transformation. Results presented here provide novel clues to reconstruct the interaction of trophoblast cells and neutrophils in vivo during placentation in humans.