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Gestational diabetes mellitus affects odontoblastic differentiation of dental papilla cells via Toll‐like receptor 4 signaling in offspring
Author(s) -
Lyu Yun,
Jia Sixun,
Wang Shikang,
Wang Tao,
Tian Weidong,
Chen Guoqing
Publication year - 2020
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.29240
Subject(s) - offspring , dental papilla , odontoblast , endocrinology , medicine , signal transduction , dentinogenesis , receptor , gestational diabetes , microbiology and biotechnology , dentin , biology , chemistry , pregnancy , pathology , gestation , genetics
Gestational diabetes mellitus (GDM) is an important factor involved in the pathogenesis of organ development in the offspring. Here, we analyzed the effects of GDM on odontoblastic differentiation of dental papilla cells (DPCs) and dentin formation in offspring and investigated their underlying mechanisms. A GDM rat model was induced by intraperitoneal injection of streptozotocin and offspring were collected. The results showed that GDM significantly affected odontoblast differentiation and dentin formation in offspring tooth. GDM activated the toll‐like receptor 4 (TLR4)/nuclear factor‐kappa B (NF‐ĸB) signaling pathway and inhibited SMAD1/5/9 signaling to modulate the odontoblastic differentiation of DPCs in offspring. Inhibition of TLR4 signaling by treated with TAK‐242 significantly reverses the suppression of odonto‐differentiation of DPCs in diabetic offspring. Taken together, these data indicate GDM activated the offspring DPCs TLR4/NF‐ĸB signaling, which suppressed the SMAD1/5/9 phosphorylation and then inhibited odontoblasts differentiation and dentin formation.