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Local anesthetics counteract cell proliferation and migration of human triple‐negative breast cancer and melanoma cells
Author(s) -
Castelli Vanessa,
Piroli Alba,
Marinangeli Franco,
d'Angelo Michele,
Benedetti Elisabetta,
Ippoliti Rodolfo,
Zis Panagiotis,
Varrassi Giustino,
Giordano Antonio,
Paladini Antonella,
Cimini Annamaria
Publication year - 2020
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.29236
Subject(s) - terminal deoxynucleotidyl transferase , cancer cell , melanoma , cancer research , breast cancer , medicine , apoptosis , cell growth , cancer , blot , signal transduction , tunel assay , biology , pathology , microbiology and biotechnology , immunohistochemistry , biochemistry , gene
In different retrospective studies, a protective role of regional anesthetics in reducing cancer recurrence after surgery was indicated. Accordingly, it has been previously demonstrated a protective effect of anesthetics in breast cancer cells and in other types of cancer. On the other hand, how anesthetics influence cancer needs in‐depth investigations. For this purpose, two different human cancer cell lines, MDA‐MB‐231, triple‐negative breast cancer, and A375, melanoma, were used in this study. By means of Western blotting and immunofluorescence and terminal deoxynucleotidyl transferase dUTP nick end labeling analyses, the signal transduction pathways activated by the anesthetics, such as ropivacaine and levobupivacaine, were analyzed. The data obtained demonstrated that both anesthetics are able to counteract cell proliferation by positively modulating cell death signaling and by decreasing cell proliferation and survival pathways.