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Melatonin protects against defects induced by malathion during porcine oocyte maturation
Author(s) -
Chen Li,
Zhang JingJing,
Zhang Xia,
Liu Xiaoli,
Zhao Shuhong,
Huo LiJun,
Zhou Jilong,
Miao YiLiang
Publication year - 2020
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.29189
Subject(s) - oocyte , lipid droplet , reactive oxygen species , melatonin , lipid peroxidation , microbiology and biotechnology , andrology , biology , oocyte activation , lipid metabolism , chemistry , oxidative stress , endocrinology , embryo , medicine
Malathion (MAL) is a common organophosphorus pesticide and affects both animal and human reproduction. However, the mechanisms regarding how MAL affects the mammalian oocyte quality and how to prevent it have not been fully investigated. In this study, we used porcine oocyte as a model and proved that MAL impaired porcine oocyte quality in a dose‐dependent manner during maturation. MAL decreased the first polar body extrusion, disrupted spindle assembly and chromosome alignment, impaired cortical granules (CGs) distribution, and increased reactive oxygen species (ROS) level in oocytes. RNA‐seq analysis showed that MAL exposure altered the expression of 2,917 genes in the porcine maturated oocytes and most genes were related to ROS, the lipid droplet process, and the energy supplement. Nevertheless, these defects could be remarkably ameliorated by adding melatonin (MLT) into the oocyte maturation medium. MLT increased oocyte maturation rate and decreased the abnormities of spindle assembly, CGs distribution and ROS accumulation in MAL‐exposed porcine oocytes. More important, MLT upregulated the expression of genes related to lipid droplet metabolism ( PPARγ and PLIN2 ), decreased lipid droplet size and lipid peroxidation in MAL‐exposed porcine oocytes. Finally, we found that MLT increased the blastocysts formation and the cell numbers of blastocysts in MAL‐exposed porcine oocytes after parthenogenetic activation, which was mediated by reduction of ROS levels and maintaining lipid droplet metabolism. Taken together, our results revealed that MLT had a protective action against MAL‐induced deterioration of porcine oocyte quality.