Premium
Aspirin inhibits osteoclast formation and wear‐debris‐induced bone destruction by suppressing mitogen‐activated protein kinases
Author(s) -
Shi Jiawei,
Wang Zhen,
Guo Xiaobin,
Shen Jining,
Sun Houyi,
Bai Jiaxiang,
Yu Binqing,
Wang Liangliang,
Zhou Wei,
Liu Yu,
Zhang Wen,
Yang Huilin,
Xu Yaozeng,
Zhou Jun,
Geng Dechun
Publication year - 2020
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.29164
Subject(s) - osteoclast , osteolysis , bone resorption , osteoprotegerin , chemistry , aspirin , kinase , cancer research , medicine , pharmacology , microbiology and biotechnology , receptor , activator (genetics) , surgery , biology , biochemistry
Excessive osteoclast recruitment and activation is the chief cause of periprosthetic osteolysis and subsequent aseptic loosening, so blocking osteolysis may be useful for protecting against osteoclastic bone resorption. We studied the effect of aspirin on titanium (Ti)‐particle‐induced osteolysis in vivo and in vitro using male C57BL/6J mice randomized to sham (sham surgery), Ti (Ti particles), low‐dose aspirin (Ti/5 mg·kg −1 ·d −1 aspirin), and high‐dose aspirin (Ti/30 mg·kg −1 ·d −1 aspirin). After 2 weeks, a three‐dimensional reconstruction evaluation using micro‐computed tomography and histomorphology assessment were performed on murine calvariae. Murine hematopoietic macrophages and RAW264.7 lineage cells were studied to investigate osteoclast formation and function. Aspirin attenuated Ti‐particle‐induced bone erosion and reduced osteoclasts. In vitro, aspirin suppressed osteoclast formation, osteoclastic‐related gene expression, and osteoclastic bone erosion in a dose‐dependent manner. Mechanically, aspirin reduced osteoclast formation by suppressing receptor activator of nuclear factor kappa‐B ligand‐induced activation of extracellular signal‐related kinase, p‐38 mitogen‐activated protein kinase, and c‐Jun N‐terminal kinase. Thus, aspirin may be a promising option for preventing and curing osteoclastic bone destruction, including peri‐implant osteolysis.