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MicroRNA‐33 and SIRT1 influence the coronary thrombus burden in hyperglycemic STEMI patients
Author(s) -
D'Onofrio Nunzia,
Sardu Celestino,
Paolisso Pasquale,
Minicucci Fabio,
Gragnano Felice,
Ferraraccio Franca,
Panarese Iacopo,
Scisciola Lucia,
Mauro Ciro,
Rizzo Maria Rosaria,
Mansueto Gelsomina,
Varavallo Federica,
Brunitto Giuseppina,
Caserta Rosanna,
Tirino Virginia,
Papaccio Gianpaolo,
Barbieri Michelangela,
Paolisso Giuseppe,
Balestrieri Maria Luisa,
Marfella Raffaele
Publication year - 2020
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.29064
Subject(s) - medicine , cardiology , myocardial infarction , thrombus , percutaneous coronary intervention , sirtuin 1 , coronary thrombosis , inflammation , downregulation and upregulation , biology , biochemistry , gene
Primary percutaneous coronary intervention (PPCI) is a pivotal treatment in ST‐segment elevation myocardial infarction (STEMI) patients. However, in hyperglycemic‐STEMI patients, the incidence of death is still significant. Here, the involvement of sirtuin 1 (SIRT1) and miR33 on the pro‐inflammatory/pro‐coagulable state of the coronary thrombus was investigated. Moreover, 1‐year outcomes in hyperglycemic STEMI in patients subjected to thrombus aspiration before PPCI were evaluated. Results showed that hyperglycemic thrombi displayed higher size and increased miR33, reactive oxygen species, and pro‐inflammatory/pro‐coagulable markers. Conversely, the hyperglycemic thrombi showed a lower endothelial SIRT1 expression. Moreover, in vitro experiments on endothelial cells showed a causal effect of SIRT1 modulation on the pro‐inflammatory/pro‐coagulative state via hyperglycemia‐induced miR33 expression. Finally, SIRT1 expression negatively correlated with STEMI outcomes. These observations demonstrate the involvement of the miR33/SIRT1 pathway in the increased pro‐inflammatory and pro‐coagulable state of coronary thrombi in hyperglycemic STEMI patients.