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MiR‐137 knockdown promotes the osteogenic differentiation of human adipose‐derived stem cells via the LSD1/BMP2/SMAD4 signaling network
Author(s) -
Ma Xiaohan,
Fan Cong,
Wang Yuejun,
Du Yangge,
Zhu Yuan,
Liu Hao,
Lv Longwei,
Liu Yunsong,
Zhou Yongsheng
Publication year - 2020
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.29006
Subject(s) - gene knockdown , microbiology and biotechnology , bone morphogenetic protein 2 , mesenchymal stem cell , microrna , stem cell , regulator , chemistry , biology , in vitro , apoptosis , biochemistry , gene
Abstract MicroRNAs are a group of endogenous regulators that participate in several cellular physiological processes. However, the role of miR‐137 in the osteogenic differentiation of human adipose‐derived stem cells (hASCs) has not been reported. This study verified a general downward trend in miR‐137 expression during the osteogenic differentiation of hASCs. MiR‐137 knockdown promoted the osteogenesis of hASCs in vitro and in vivo. Mechanistically, inhibition of miR‐137 activated the bone morphogenetic protein 2 (BMP2)‐mothers against the decapentaplegic homolog 4 (SMAD4) pathway, whereas repressed lysine‐specific histone demethylase 1 (LSD1), which was confirmed as a negative regulator of osteogenesis in our previous studies. Furthermore, LSD1 knockdown enhanced the expression of BMP2 and SMAD4, suggesting the coordination of LSD1 in the osteogenic regulation of miR‐137. This study indicated that miR‐137 negatively regulated the osteogenic differentiation of hASCs via the LSD1/BMP2/SMAD4 signaling network, revealing a new potential therapeutic target of hASC‐based bone tissue engineering.