Premium
Slc25a17 acts as a peroxisomal coenzyme A transporter and regulates multiorgan development in zebrafish
Author(s) -
Kim YongIl,
Nam InKoo,
Lee DongKyu,
Bhandari Sushil,
Charton Lennart,
Kwak SeongAe,
Lim JaeYoung,
Hong KwangHeum,
Kim SeJin,
Lee Joon No,
Kwon Sung Won,
So HongSeob,
Linka Nicole,
Park Raekil,
Choe SeongKyu
Publication year - 2020
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.28954
Subject(s) - zebrafish , peroxisome , gene knockdown , morpholino , biology , danio , microbiology and biotechnology , function (biology) , transporter , peroxisomal targeting signal , gene , biochemistry
Slc25a17 is known as a peroxisomal solute carrier, but the in vivo role of the protein has not been demonstrated. We found that the zebrafish genome contains two slc25a17 genes that function redundantly, but additively. Notably, peroxisome function in slc25a17 knockdown embryos is severely compromised, resulting in an altered lipid composition. Along the defects found in peroxisome‐associated phenotypic presentations, we highlighted that development of the swim bladder is also highly dependent on Slc25a17 function. As Slc25a17 showed substrate specificity towards coenzyme A (CoA), injecting CoA, but not NAD + , rescued the defective swim bladder induced by slc25a17 knockdown. These results indicated that Slc25a17 acts as a CoA transporter, involved in the maintenance of functional peroxisomes that are essential for the development of multiple organs during zebrafish embryogenesis. Given high homology in protein sequences, the role of zebrafish Slc25a17 may also be applicable to the mammalian system.