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MiR‐210‐3p inhibits osteogenic differentiation and promotes adipogenic differentiation correlated with Wnt signaling in ERα‐deficient rBMSCs
Author(s) -
Li Xiaoyun,
Peng Bojia,
Zhu Xiaofeng,
Wang Panpan,
Sun Kehuan,
Lei Xiaotong,
He Haibin,
Tian Ya,
Mo Shu,
Zhang Ronghua,
Yang Li
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.28916
Subject(s) - adipogenesis , wnt signaling pathway , microrna , microbiology and biotechnology , downregulation and upregulation , signal transduction , cellular differentiation , chemistry , biology , mesenchymal stem cell , gene , biochemistry
MicroRNAs (miRNAs) regulate activities in living organisms through various signaling pathways and play important roles in the development and progression of osteoporosis. The balance between osteogenic and adipogenic differentiation of rBMSCs is closely related to the occurrence of osteoporosis. ERα regulates bone metabolism in various tissues. However, the correlation among ERα, miRNAs, and the differentiation of rBMSCs is still unclear. In this study, we used lentivirus transfection into rBMSCs to construct an ERα‐deficient model, analyzed the differences in expressed miRNAs between control and ERα‐deficient rBMSCs. The results revealed that the expression of 25 miRNAs were upregulated, 164 miRNAs were downregulated, and some of the regulated miRNAs such as miR‐210‐3p and miR‐214‐3p were related to osteogenic or adipogenic differentiation, as well as to particular signaling pathways. Next, we overexpressed miR‐210‐3p to evaluate its effects on the osteogenic and adipogenic differentiation of rBMSCs, and identified the relationship among miR‐210‐3p, Wnt signaling pathway, and the differentiation of rBMSCs. The results indicated that ERα‐deficient inhibited osteogenic differentiation, promoted adipogenic differentiation, and regulated the expression of some miRNAs. Meanwhile, overexpression of miR‐210‐3p promoted osteogenic differentiation and inhibited adipogenic differentiation of rBMSCs, processes likely to be related to the Wnt signaling pathway. In conclusion, we identified a group of upregulated and downregulated miRNAs in ERα‐deficient rBMSCs that might play a vital role in regulating osteogenic or adipogenic differentiation. One of these, miR‐210‐3p, inhibited osteogenic differentiation and promoted adipogenic differentiation correlated with the Wnt signaling pathway in ERα‐deficient rBMSCs, providing new insight into the regulation of bone metabolism.